Coding

Part:BBa_K1973036:Design

Designed by: Laura Claret Fernndez   Group: iGEM16_UPO-Sevilla   (2016-10-09)


lapC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 643
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 533
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have included the sequence of RBS BBa_B0034 by means of the PCR primers, as well as the scar it should have been in case of the clonning of this part. It had to be site-directed mutagenized in position 552 in order to remove a PstI restriction site.

Source

It was obtained by synthesis.

References